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. 2017 Feb 6;24(1):300–308. doi: 10.1080/10717544.2016.1247927

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — The characteristics of endostatin-loaded nanoparticles. (A) The fabrication process of ES-NPs. Endostatin-loaded chitosan nanoparticles were prepared by ionic cross-linking method with dropwise addition of TPP to a chitosan solution. (B) The size distribution of our chosen ES-NPs. The results showed that the particles were 246.89 ± 3.5 nm in diameter. (C) The release behavior of ES-NPs in vitro. The endostatin release profile was biphasic, with an initial abrupt release and a subsequent sustained release. (D) The formulation stability of ES-NPs in mouse serum at 4 °C, 37 °C or room temperature (RT). (E) TEM images of ES-NPs. Transmission electron microscopy showed that nanoparticles were round particles with relative smooth edges.