Figure 1.

Distribution of pDNA in tissue sections of the suctioned kidney determined by tissue section and tissue clearing using Scale SQ reagent. (A) Ten micrograms of Cy5-labeled pCMV-Luc was injected, and then the right kidney was suctioned at −30 kPa. Sham-operated mice were injected with the same amount of Cy5-labeled pCMV-Luc but not suctioned. After 10 min, frozen sections were prepared and stained with DiI and DAPI. The stained sections were observed by confocal laser scanning microscopy (CLSM). Magnification: ×40. Left: sham-operated group; right: renal suction group. Green: Cy5-labeled pCMV-Luc; red: DiI-stained cytoskeleton; blue: nucleus. White arrows show the uptake of Cy5-labeled pCMV-Luc to the cytoplasm of interstitial cells. All scale bars represent 50 μm. (B) Ten micrograms of Cy5-labeled pZsGreen1-N1 was injected, and then the right kidney was suctioned at −30 kPa. Sham-operated mice were injected with the same amount of Cy5-labeled pZsGreen1-N1 but without suction. After 10 min, mice were perfused with a DiI solution to stain vascular vessels. Then, the collected kidney was cleared by immersion in Scale SQ reagent. The stained kidney was observed by CLSM. Magnification: ×40. Left: Sham-operated group; right: renal suction group. Green: Cy5-labeled pZsGreen1-N1; red: vascular vessel stained by DiI; blue: nucleus. All scale bars represent 50 μm. (C) Cy5-labeled pZsGreen1-N1 was injected in the same manner as described in (B). After 24 h, mice were perfused with the DiI solution to stain vascular vessels. Then, the collected kidney was cleared by immersion in Scale SQ reagent. Magnification: ×40. Yellow: ZsGreen1; cyan: Cy5-labeled pZsGreen1-N1; magenta: vascular vessel stained by DiI; blue: nucleus. White arrows of sham-operated show Cy5-labeled pZsGreen1-N1 merged with vascular vessel. White arrows of renal suction show Cy5-labeled pZsGreen1-N1 merged and ZsGreen1 expression with nucleus. Color version of this figure is available Online.