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. 2017 Jun 6;24(1):906–917. doi: 10.1080/10717544.2017.1333171

Figure 2.

Figure 2.

Evaluation of the transgene expression site by X-gal staining and immunostaining of frozen suctioned kidney sections. (A) The right kidney was suctioned at −30 kPa after i.v. injection of pCpG-free-LacZ. Sham-operated mice were injected with the same amount of pCpG-free-LacZ but not suctioned. The kidney was collected at 24 h after transfection, and 10 μm-thick frozen sections were stained with X-gal reagent. Left: sham-operated group; right: renal suction group. Magnification: ×20. All scale bars represent 50 μm. (B) The right kidney was suctioned at −30 kPa after i.v. injection of pZsGreen1-N1. The kidney was collected at 24 h after transfection, and 10 μm-thick frozen sections were stained with an anti-CD31 antibody and DAPI. The stained sections were observed by CLSM. Magnification: ×40. Red: CD31-positive (+) endothelial cell; green: ZsGreen1; blue: nucleus. All scale bars represent 50 μm. Color version of this figure is available Online.