Skip to main content
. 2017 Jun 6;24(1):906–917. doi: 10.1080/10717544.2017.1333171

Figure 5.

Figure 5.

Influence of cellular uptake and transcription processes on transgene expression using the renal suction method. (A) The right kidney was suctioned at −30 kPa at various times after i.v. injection of pCMV-Luc [from before (−) 60 to after 180 s]. Transgene expression levels in the kidney were determined by a luciferase assay at 6 h after transfection. Mice in the sham-operated group (control) were injected with the same amount of pCMV-Luc as mice in the suctioned groups. Each bar represents the mean ± SD (n = 4–5). *p < 0.05. (B) The right kidney was suctioned at −30 kPa immediately after i.v. injection of each pDNA (pAP-1-Luc, pNF-κB-Luc, or pTAL-Luc). Transgene expression levels in the kidney were determined by a luciferase assay at 6 h after transfection. Each bar represents the mean ± SD (n = 6–9). Abbreviations: TAL, pTAL-Luc; AP-1, pAP-1-Luc; NF-κB, pNF-κB-Luc administration groups. **p < .01 vs. pTAL-Luc administration group. (C and D) The right kidney was suctioned at −30 kPa immediately after i.v. injection of pCMV-Luc. The mRNA levels of (C) c-fos and (D) c-jun in the kidney were determined by real-time PCR at 1, 3, and 6 h after transfection. mRNA levels were normalized to the mRNA level of GAPDH in each sample (n = 3–5).