Figure 4.

Effect of CPT and CN-CPT on cells adhesion and cell migration of CAL-62 and BHT-101 cell lines. (A,B) HUVEC were treated or not treated with CPT or CN-CPT for 24 h, washed and used in the adhesion assay with untreated CAL-62 (A) and BHT-101 (B) cells (1 × 10⁵/well). The data are presented as percentage of inhibition of the adhesion of treated cells compared to control (untreated cells). Each experiment was performed in triplicate. Data shown are means ± SEM (n = 5). *p < 0.05; **p < 0.01 significantly different from the same concentration of CPT. (C,D) In the Boyden chamber assay, CAL-62 (C) and BHT-101 (D) cells were plated onto the apical side of Matrigel-coated filters in the presence and absence of either CPT or CN-CPT, and FCS 20% was loaded in the basolateral chamber as a chemotactic stimulus. Data are expressed as mean ± SEM (n = 5) of the percentage of inhibition versus control migration **p < 0.01 significantly different from the same concentrations of CPT.