Figure 5.
Effect of CPT and CN-CPT on β-PIX expression and Erk1,2 phosphorylation in CAL -62 (A), and BHT-101 (C). Cells were treated with CPT or CN-CPT (10−8 and 10−9 M) for 48 h. CPT and CN-CPT were replenished every 24 h (48 h culture: 24 + 24 h) without changing the culture medium. The same blots were probed with anti β-actin antibody as a control. (A, C): Western blot analysis from a representative experiment. (B, D): Densitometric analysis of β-PIX expression and Erk1,2 phosphorylation expressed in arbitrary units; data are expressed as means ± SEM (n = 3) and shown as % of the controls. §p < 0.05 significantly different from untreated cells; *p < 0.05 significantly different from treated cells at the same concentration.