Figure 6.

Treatment with CN-CPT delays the growth of orthotopic anaplastic thyroid carcinoma xenografts in vivo. CAL-62 cells (1 × 10⁶) were injected into the right thyroid lobe of NSG mice. Mice were injected with PBS (○, n = 5), CPT (•, n = 7) or CN-CPT (□, n = 7) at day 10 after tumor challenge. (A) Kaplan–Meier survival analysis of untreated and treated mice. *p < 0.05 compared to the PBS-treated group. Graphs show: (B) tumor velocity and (C) the number of metastasis in the lung for each group, expressed as median and interquartile range. *p < 0.05; ***p < 0.001 compared to the PBS-treated group and CPT-treated group. (D) Representative immunohystochemical staining for Ki-67 of tumor sections from mice treated with PBS, CPT or CN-CPT sacrificed at day 34 after tumor challenge. (E) Ki-67 staining of tumor section from a mouse treated with CN-CPT and sacrificed at day 51 after tumor challenge. (F) Graph shows the tumor microvessel density (TMD) determined as the percentage of CD31-positive area on the tumor sections. Three randomly selected areas from three tumors from each group were analyzed; data are expressed as median and interquartile ranges. ^***p < 0.001 compared to the PBS-treated group and CPT-treated group.