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. 2017 Jun 20;24(1):952–961. doi: 10.1080/10717544.2017.1337827

Figure 4.

Figure 4.

Restoration of dystrophin in TA muscles of mdx mice (aged 4–5 weeks) 2 weeks after i.m. injection. [The samples were from muscles treated with PQs (20 μg) and PMOE23 (2 μg) in 40 μL saline, PMOE23 only treated as controls]. (A) Dystrophin was detected by immunohistochemistry with rabbit polyclonal antibody P7 against dystrophin. Blue nuclear staining with DAPI, and original magnification: ×100. (B) The percentage of dystrophin-positive fibers in muscles treated with PQs formulated with PMOE23 (n = 5, two-tailed t-test, *p ≤ .05 compared with PMO alone). (C) Detection of exon 23 skipping by RT-PCR. Total RNA of 100 ng from each sample was used for amplification of dystrophin mRNA from exon 20 to exon 26. The upper bands (1093 bp, indicated by E22 + E23 + E24) correspond to the normal mRNA, and the lower bands (880 bp, indicated by E22 + E24) correspond to the mRNA with exon E23 skipped. (D) Western blots demonstrate the expression of dystrophin protein. Dys, dystrophin detected with monoclonal antibody Dys 1. α-Actin was used as loading control.