FIGURE 6.

Intrathecal CpG induced recruitment of monocytes to the CNS and reduced demyelination in experimental autoimmune encephalomyelitis (EAE). (A) Flow cytometry profiles of mice with EAE treated with intrathecal CpG or control (ctrl). CD45high leukocyte populations are distinguished from CD45dim microglia. (B) Quantitative flow cytometric analysis comparing the percentage of CD45+ leukocytes, (CD45^hiCD11b^hiGR1^low/– F4/80⁺) monocytes, neutrophils (CD45^hiCD11b^hiGR1^hiF4/80^–), and CD4+ T cells (CD45^hiCD11b^low/– TCRβ⁺CD4⁺) (n = 6–8 per group). Results were analyzed using the two-tailed Mann–Whitney U-test. (C) Representative images of spinal cord sections of mice with EAE stained with H&E and anti-MOG antibody. Boxes show selected areas of the spinal cord sections with higher magnification. Arrows highlight infiltration or loss of MOG. Scale bars: 200 and 100 μm. Data are presented as means ± SD. **p < 0.01.