Figure 3.

(A) Normalized fluorescence autocorrelation data obtained from free Rh110 in solution (black solid line), 140 nM Rh110-labeled SBP1 after 30 min (gray solid line) and 24 h (dark gray dotted line) of incubation. (B) Autocorrelation traces obtained from 3 nM Rh110-labeled SBP1 at time t = 0 (black solid line) and after 20 min (black dashed line), 40 min (gray, solid line), and 1 h (black, dotted line) of incubation. (C) R_h-values determined from the FCS experiments for Rh110-labeled SBP1 and SBP1^mod at 140 nM after vortexing for 30 min (white) and after 24 h (gray). The error bars are plotted as SEs of the mean (n = 5). (D) R_h of Rh110-labeled SBP1 (square) and SBP1^mod (circle) as a function of time at a final peptide concentration of 3 nM. The error bars are plotted as SEs of the mean (n = 10 for SBP1, and n = 8 for SBP1^mod). Solid lines are guide to the eye.