Figure 2.
Effect of famotidine on 3CLPro activity and viral replication.A, 3CLpro protein was preincubated with varying concentration of famotidine, followed by the addition of protein substrate as described in Experimental procedures section. Famotidine showed no inhibitory effect on CoV-2 3CLpro, when compared with positive control, Boceprevir, which inhibited the 3CLpro protease with an IC50 value of 2.3 μM. B, effect of famotidine and remdesivir on virus-infected cells after 24 h of infection, where remdesivir or famotidine was added at the time of infection. Spike positive area was measured 24 h after infection. Error bars indicate SD from three experiments. ∗∗∗p < 0.005. Scale bars: 100 μm.