Fig. 5.

(A) Images and histology using H&E of the chick embryo chorioallantoic membrane (CAM) for vehicle control, positive control (Sunitinib) and test compounds. At day 8, eggs were opened carefully. Twenty μl of control vehicle or test compounds (final concentration of 660 μM) were left to dry on a 2.5 cm round glass slide on the surface of the CAM blood network. Eggs were covered and left in an incubator at 37 °C for 3 days. Later the membrane around the area of interest was fixed with 5% formalin, and paraffin embedded for further staining. Original images were done at 40x and 100x magnification “CM: chorionic membrane. ML: mesodermal layer. BV: blood vessel”. (B) Quantitative analysis of blood vessel diameter in mm after 3 days of treatment. Sunitinib (positive control) and test compounds all show significant reduction in BV diameters. All results are expressed as mean ± SD (n = 4) (***P < 0.001 in comparison to control).