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Spike pseudotyped lentiviral infectivity assay, related to Figure 2
(A) Flow cytometry plots showing %ZsGreen-positive 293T and 293T-ACE2 cells after 60h incubation with ZsGreen backbone lentiviruses pseudotyped with no Spike protein (delta Spike; gray), wild-type (WT) Spike protein (green) or VSV-G (black) envelope protein. Composite pseudotyped lentiviral infectivity data of (B) 293T or (C) 293T-ACE2 cells at five-fold and two-fold dilutions of neat stock virus preparations.
