Fig. 2.

Chronic As³⁺ exposure changes the landscape of differentially expressed miRNAs and mRNAs in a longitudinal manner. A Bar graph showing the number of miRNA molecules at 7, 19 and 28-wk time points differentially expressed in HaCaT cells exposed to 100 nM As³⁺ compared with passage matched unexposed controls (induced: closed bars; suppressed: open bars). Differential expression is defined as p_Eq < 0.05. B Venn diagram depicting the distribution of differentially expressed miRNAs (p_Eq < 0.05) at each time point along with the number of overlaps at different time points. C Heat Map of differentially expressed miRNA molecules at 7, 19 and 28-wk. The numerals on the y axis refers to the serial numbers assigned to the differentially expressed miRNA molecules in Supplementary Table 2. The color code bar on top refers to the log₂ (Fold Change) expression values. Absence of a bar (represented by white) signifies either that miRNA molecule was not detected at that time point or was not differentially expressed at that time point. D Bar graph showing the number of mRNA molecules at 7, 19 and 28-wk time points differentially expressed in HaCaT cells exposed to 100 nM As³⁺ compared with passage matched unexposed controls (induced: closed bars; suppressed: open bars). Differential expression is defined as p < 0.01 and FC ±  > 30%. E Venn diagram depicting the distribution of differentially expressed mRNAs (p < 0.01 and FC ±  > 30%) at each time point along with the number of overlaps at different time points. F Heat Map of differentially expressed mRNA molecules at 7, 19 and 28-wk. The numerals on the y axis refers to the serial numbers assigned to the differentially expressed miRNA molecules in Supplementary Table 3. The color code bar on top refers to the log₂ (Fold Change) expression values. Absence of a bar (represented by white) signifies either that mRNA molecule was not detected at that time point or was not differentially expressed at that time point. G Expression pairing between differentially expressed miRNA and differentially expressed mRNA at 7, 19 and 28-wk. For each time point, shown are the total number of differentially expressed miRNA molecules that are targeting one or more differentially expressed mRNA molecules (light grey bars); the number of differentially mRNA molecules that are targeted by one or more or differentially expressed miRNA molecules (closed bars), total number of miRNA-mRNA pairings (open bars) and the number of differentially expressed mRNA molecules that are not predicted to be targeted by any differentially expressed miRNA molecule (hatched bars). h Concordance–discordance relationship between differentially expressed miRNA and differentially expressed mRNA at 7, 19 and 28-wk. The data is presented as % of total interactions predicted at that time point