. 2021 Jun 1;17(9):2205–2222. doi: 10.7150/ijbs.58295

Table 2.

Infiltrated levels of immune cell signatures with HK3 mRNA expression in ccRCC

Description	Gene markers	HK3 expression
		None		Purity
		Cor.	P	Cor.	P
CD8+ T cell	CD8A	0.382	********	0.335	********
	CD8B	0.382	********	0.34	********
T cell (general)	CD3D	0.428	********	0.386	********
	CD3E	0.422	********	0.373	********
	CD2	0.420	********	0.373	********
B cell	CD19	0.384	********	0.353	********
	CD79A	0.363	********	0.323	********
Monocyte	CD86	0.551	********	0.518	********
	CD115 (CSF1R)	0.529	********	0.507	********
TAM	CCL2	0.088	*	0.017	0.714
	CD68	0.450	********	0.466	********
	IL10	0.511	********	0.486	********
M1 Macrophage	INOS (NOS2)	-0.07	0.107	-0.119	*
	IRF5	0.435	********	0.440	********
	CD80	0.593	********	0.593	********
	COX2 (PTGS2)	0.061	0.158	0.017	0.721
M2 Macrophage	CD163	0.51	********	0.512	********
	VSIG4	0.597	********	0.598	********
	MS4A4A	0.521	********	0.506	********
Neutrophils	CD66b (CEACAM8)	0.101	*	0.121	**
	CD11b (ITGAM)	0.627	********	0.604	********
	CCR7	0.373	****	0.336	****
Natural killer cell	KIR2DL1	0.107	*	0.065	0.164
	KIR2DL3	0.103	*	0.086	0.065
	KIR2DL4	0.225	****	0.193	****
	KIR3DL1	0.059	0.172	0.056	0.23
	KIR3DL2	0.091	*	0.058	0.211
	KIR3DL3	0.112	**	0.098	*
	KIR2DS4	0.05	0.248	0.012	0.805
Dendritic cell	HLA-DPB1	0.422	********	0.397	****
	HLA-DQB1	0.267	****	0.228	****
	HLA-DRA	0.422	********	0.400	****
	HLA-DPA1	0.380	****	0.346	****
	BDCA-1 (CD1C)	0.212	****	0.156	***
	BDCA-4 (NRP1)	-0.098	*	-0.151	**
	CD11c (ITGAX)	0.698	********	0.683	********
Th1	T-bet (TBX21)	0.374	****	0.341	****
	STAT4	0.412	****	0.376	****
	STAT1	0.340	****	0.295	****
	IFN-γ (IFNG)	0.420	****	0.377	****
	TNF-α (TNF)	0.387	****	0.361	****
Th2	GATA3	0.122	**	0.095	*
	STAT6	0.196	****	0.233	****
	STAT5A	0.438	********	0.418	********
	IL13	0.205	****	0.197	****
Tfh	BCL6	0.175	****	0.191	****
	IL21	0.197	****	0.181	****
Th17	STAT3	0.084	0.052	0.063	0.177
	IL17A	0.066	0.126	0.03	0.521
Treg	FOXP3	0.459	********	0.425	********
	CCR8	0.386	****	0.357	****
	STAT5B	-0.09	*	-0.095	0.041
	TGFβ (TGFB1)	0.16	***	0.123	**
T cell exhaustion	PD-1 (PDCD1)	0.483	********	0.455	********
	CTLA4	0.475	********	0.437	********
	LAG3	0.47	********	0.432	********
	TIM-3 (HAVCR2)	0.190	****	0.152	**
	GZMB	0.377	****	0.320	****

TAM, tumor-associated macrophage; Th, T helper cell; Tfh, Follicular helper T cell; Treg, regulatory T cell; Cor, R value of Spearman's correlation; None, correlation without adjustment. Purity, correlation adjusted by purity; * P< 0.05; ** P< 0.01; *** P< 0.001; **** P< 0.0001; Cor. Value higher than 0.4 was considered as statistically significance and marked in bold.