Figure 4.

Kindling by repeated PTZ administration promotes DAPK1 protein expression in mice. Mice (C57BL/6, male, P56-P80, n = 8/group) were treated with a subconvulsive dose of PTZ (35 mg/kg) every other day (four injections for 7 days), and brain tissues were harvested 30 min after the fourth PTZ injection. A Immunohistochemistry using an anti-DAPK1 antibody was conducted on paraffin-embedded cortical and hippocampal sections from the control and PTZ-treated mice. Scale Bar = 100 µm. B Quantitation of DAPK1 staining intensity (arbitrary units; two-tailed Student's t test). C Correlation between the Racine score on the Y axis and DAPK1 expression on the X axis (R² = 0.9525, P < 0.01, Pearson correlation coefficient). D-G Cortical and hippocampal lysates were analyzed via immunoblotting with an anti-pSer308-DAPK1, anti-DAPK1 or anti-β-actin antibodies (^***P < 0.001 vs control; two-tailed Student's t tests). All data shown represent the means ± standard errors of three independent experiments.