Table 2.
Primary antibodies (A) and secondary (B) employed in immunochemistry studies, showing dilutions used and protocol specifications.
| A | Antigen | Species | Dilution | Dealer | Protocol specifications |
|---|---|---|---|---|---|
| IGF-1 | Rabbit | 1:100 | Abcam (ab263903) | Sodium citrate 10 mM pH = 6 before incubation with blocking solution. | |
| PAPP-A | Mouse | 1: 500 | Abcam (ab52030) | Triton 0,1% in PBS, 10 minutes, before incubation with blocking solution. | |
| STC-2 | Rabbit | 1:300 | Abcam (ab261915) | -------------------- | |
| Wnt-1 | Rabbit | 1:50 | Abcam (ab63934) | Triton 0,1% in PBS, 10 minutes, before incubation with blocking solution. | |
| β-catenin | Mouse | 1:100 | Abcam (ab231305) | -------------------- | |
| Cyclin D1 | Rabbit | 1:300 | Abcam (ab5262) | -------------------- | |
| c-myc | Rabbit | 1:250 | Abcam (ab32072) | -------------------- | |
| IRS-4 | Rabbit | 1:50 | Abcam (ab5262) | Triton 0,1% in PBS, 10 minutes, before incubation with blocking solution. | |
| PI3K | Mouse | 1:500 | Abcam (ab86714) | -------------------- | |
| Akt | Rabbit | 1:1000 | Abcam (ab8805) | -------------------- | |
| mTOR | Rabbit | 1:500 | Abcam (ab1093) | -------------------- | |
| IL-4 | Mouse | 1:250 | Abcam (ab239508) | Triton 0,1% in PBS, 10 minutes, before incubation with blocking solution. | |
| IL-6 | Mouse | 1:200 | Abcam, (ab9324) | Sodium citrate 10 mM pH = 6 before incubation with blocking solution. | |
| B | Antigen | Species | Dilution | Dealer | Protocol specifications |
| IgG (Mouse) | Goat | 1:300 | Sigma (F2012/045K6072 ) | -------------------- | |
| IgG (Rabbit) | Mouse | 1:1000 | Sigma (RG-96/ B5283) | -------------------- |