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. 2021 May 29;95(7):2413–2430. doi: 10.1007/s00204-021-03075-3

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© The Author(s) 2021

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Fig. 5 — Comparison of relative protein expression levels by iTRAQ and immunoblotting. a Log2 fold difference in expression of DMET and NRF2 proteins detected by iTRAQ. b Immunoblot of DMET and NRF2 proteins in liver and hepatic organoids at various stages of differentiation (n = 2). c Densitometric analysis of DMET and NRF2 proteins relative to GAPDH showed a similar protein expression to that observed by iTRAQ analysis. differentiated v liver, undifferentiated v liver, differentiated v undifferentiated (color figure online)

Inline graphic — Comparison of relative protein expression levels by iTRAQ and immunoblotting. a Log2 fold difference in expression of DMET and NRF2 proteins detected by iTRAQ. b Immunoblot of DMET and NRF2 proteins in liver and hepatic organoids at various stages of differentiation (n = 2). c Densitometric analysis of DMET and NRF2 proteins relative to GAPDH showed a similar protein expression to that observed by iTRAQ analysis. differentiated v liver, undifferentiated v liver, differentiated v undifferentiated (color figure online)