Figure 4.

Centella extracts prevent H₂O₂-induced cytotoxicity in dermal fibroblasts. (A) and (B) CE and APE at 15–60 µg/mL did not cause toxicity in dermal fibroblasts. BJ cells were incubated with CE or APE (15–60 µg/mL) for 24 h. (C) and (D) CE and APE inhibit oxidative damage from H₂O₂ exposure. BJ cells were pre-incubated with CE or APE (15–60 µg/mL) for 24 h then exposed to H₂O₂ (200 µM) for 1 h. The cell viability was evaluated with MTT assay immediately after the treatments. (E) and (F) CE inhibit depletion of intracellular ATP following H₂O₂ exposure. The protocol for treatment was as (A) and (C). The amounts of ATP were measured immediately after treatments (n = 3; mean ± SEM; *P < 0.05 vs untreated control; ^†P < 0.05 vs H₂O₂-treated cells).