Figure 7.

Centella extracts inhibit expression of MMP9 following H₂O₂ treatment. (A) Exposure to H₂O₂ resulted in elevation of MMP9 expression, while CE and APE did not affect the transcription of MMP9. BJ cells were treated CE (15–60 µg/mL; 24 h) or APE (60 µg/mL; 24 h) or H₂O₂ (200 µM; 1 h). (B) CE and APE suppressed H₂O₂-induced MMP9 expression. BJ cells were pre-incubated with CE or APE (60 µg/mL) for 24 h then exposed to H₂O₂ (200 µM) for 1 h. The transcriptional levels of MMP9 were evaluated with RT-PCR following treatments (n = 3; mean ± SEM; *P < 0.05 vs untreated control; ^†P < 0.05 vs H₂O₂-treated cells).