Table 1.
Bacterial strains and plasmids used in this study.
| Strains, vectors and primers | Genotypes or description | Origin |
|---|---|---|
| Bacterial stain | ||
| TOP10 | F- Φ80lacZΔM15 ΔlacX74 recA1 | Invitrogen |
| Vectors | ||
| pET-21a | AmpR, T7 promoter, lac operator | Novagen |
| pET-vio | pET-21a derivative containing the violacien expression cassette (vioABCDE) inserted as a NdeI/SacI fragment | 22 |
| pPmer | pET-21a derivative containing merR and Pmer divergent promoter region cloned into BglII and XbaI sites | 42 |
| pPmer-R-Pmer-G | pPmer derivative, an artificial hybrid mer operon with transcriptions of mcherry and egfp under the control of independent Pmer divergent promoter region | 42 |
| pPmer-vio | pET-vio derivative containing merR and Pmer divergent promoter cloned as a BglII/XbaI fragment | This study |
| pPmer-vio-Pmer-G | pPmer derivative, an artificial hybrid mer operon with transcriptions of vioABCDE and egfp under the control of independent Pmer divergent promoter region | This study |
| pPmer-G | pPmer derivative carrying promoterless egfp cloned into NdeI and HindIII sites | This study |
| Primers | ||
| F-mer | GAAGATCTCTAAGGCATAGCTGACC | This study |
| R-mer | GCTCTAGAACGTTGGCCCTTTTG | This study |
| F-Pmer-G | AAGAGCTCATCGCTTGACTCCGTAC | This study |
| R-Pmer-G | ATAAGAATGCGGCCGCTTATTTGTACAGTTCATCCATAC | This study |
| F-G | GGAATTCCATATGGTTTCTAAAGGCG | This study |
| R-G | CCCAAGCTTTTATTTGTACAGTTCATCCATAC | This study |