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. 2021 Jun 29;11:13460. doi: 10.1038/s41598-021-92601-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Selection of LOD score limit (Lod) to construct genetic map in LepMap3. (A) The average of number of markers (nMarkers) positioned in linkage groups (left Y axis) and the number of linkage groups (nLG; right Y axis) for Lod values from 1 to 15 as implemented in the "SeparateChromosomes” module. Lod11 (shaded) indicates the value selected that grouped the markers in 21 LGs. (B) Average number of markers recovered and added to the 21 LGs using decreasing LOD score iterations from 10 to 5 in the JoinSingles2 module.