. 2021 May 20;100(8):101264. doi: 10.1016/j.psj.2021.101264

Table 1.

Primers for qRT-PCR of MyHC-related genes.

Gene	GenBank accession	Primer sequence (5′→3′)	Length (bp)	Product size (bp)	Annealing temperature (°C)
MYH7B	NM_204587.2	F: GCTGCGGTGTAACGGTGTC	19	119	60
MYH7B	NM_204587.2	R: CTGGAATGGCTGCTGGGT	18	119	60
MYH1A	NM_001013396.1	F: GAACCCTCCCAAGTATGA	18	124	60
MYH1A	NM_001013396.1	R: GAGACCCGAGTAGGTGTAG	19	124	60
MYH1B	NM_204228.3	F: GGGAGACCTGAATGAAATGGAG	22	140	60
MYH1B	NM_204228.3	R: CTTCCTGTGACCTGAGAGCATC	22	140	60
β-actin	EF667345.1	F: GAGAAATTGTGCGTGACATCA	21	152	60
β-actin	EF667345.1	R: CCTGAACCTCTCATTGCCA	19	152	60

Note: All the primers are designed with Primer 5.0 software and synthesized by Nanjing Qingke bioengineering company. F, forward primer. R, reverse primer.

Abbreviations: MyHC, myosin heavy-chain; MYH7B, (type I) slow-twitch myosin heavy-chain; MYH1A, (type IIb) fast-twitch myosin heavy-chain; MYH1B, (type IIa) fast-twitch myosin heavy-chain.