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. 2021 Mar 12;100(8):101099. doi: 10.1016/j.psj.2021.101099

Figure 2.

Figure 2

IFA and PCR results of CEF cell cultures infected with the samples. Positive fluorescence was observed in the GX18NNR1, GX19YLR1, GX19NNR1, and GD20R1 infected cells by the IFA with REV-specific monoclonal antibody 11B118 (A–D), while no specific fluorescence was observed in the non-infected cells (E). Positive fluorescence was observed in the GX18NNM7, GX19YLM5, GX19NMNM3 and GD20M1 infected cells by the IFA with MDV-1 gB-specific monoclonal antibody BA4 (F–I). No specific fluorescence was observed in the non-infected cells (J). (K) PCR was used to identify the REV and MDV positive samples. M, Marker, DL2000; RPC, CEF cells infected with GD1210 as positive control; RNC, uninfected CEF cells used as a negative control; 1-4, positive PCR products obtained using the primers specific for REV LTR of GX18NNR1, GX19YLR1, GX19NNR1 and GD20R1, respectively; MPC, CEF cells infected with GX18NNM4 as positive control; MNC, uninfected CEF cells used as a negative control; 5-8, positive PCR products obtained using the primers specific for MDV meq of GX18NNM7, GX19YLM5, GX19NMNM3 and GD20M1, respectively.