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Biofilm inhibition after treatment of AgNPs in E. coli (A) and P. aeruginosa (B). Strains were incubated for 24 h in the presence of AgNPs. Post-treatment surface-associated biofilm was stained and the OD of biofilm biomass were presented. Mean values with standard deviation (error bars) with *, **, ***are statistically different from the respective control at P < 0.05, P < 0.01, and P < 0.001, respectively (one-way ANOVA, Tukey test).
