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. 2021 Jun 29;11:13464. doi: 10.1038/s41598-021-92941-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SARS-CoV-2 protein expression in HeLa cells. (A) Genome structure of SARS-CoV-2. The ORF1a/1ab encodes non-structure proteins, four genes encode four structural proteins (S, E, M, N), and six accessory genes encoding six accessory proteins (ORF3a, ORF6, ORF7a, ORF7b, ORF8, and ORF10) are indicated. (B) SARS-CoV-2 ORF3a, M, 7a, N genes were fused with a FLAG-tag (F) at the N-terminus and cloned in the pXJ41 expression vector. pF-ORF3a, pF-M, pF-ORF7a, and pF-N were transfected in HeLa cells for 24 h, and cell lysates were subjected to immunoblot using α-FLAG PAb. Beta-actin served as a loading control. The full-length blot is presented in Supplementary Fig. S1. (C) Distribution of SARS-CoV-2 ORF3a, M, ORF7a, and N protein in HeLa cells. Cellular localization of the individual protein (red) and cell nuclei (blue) were examined by confocal microscopy.