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Inflammatory cytokine responses to SARS-CoV-2 ORF7a protein. HeLa cells were transfected with 2 μg of indicated genes for 24 h and treated with or mock-treated with TNF-α (20 ng/ml) for 6 h. The expression levels of (A) cytokines and (B) chemokines were calculated with 2−ΔΔCT method by normalizing to that of GAPDH. The fold changes were calculated with respect to the level of pXJ41. Error bars mean ± s.d. (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.
