Table 4.
Antibodies used in TIM4-ELISA or western blot.
| Antibody, clone | Manufacturer, catalog # | Working concentration |
|---|---|---|
| TIM4-ELISA | ||
| Human CD9, HI9a | BioLegend, 312102 | × 1000 |
| Human CD63, H5C6 | BioLegend, 353039 | × 1000 |
| Human CD81, 5A6 | BioLegend, 349502 | × 1000 |
| Mouse CD9, MZ3 | BioLegend, 124802 | × 1000 |
| Mouse CD63, NVG-2 | BioLegend, 143902 | × 1000 |
| Mouse/rat CD81, Eat-2 | BioLegend, 104901 | × 1000 |
| Anti-mouse IgG HRP | BioLegend, 405306 | × 5000 |
| Anti-rat IgG HRP | BioLegend, 405405 | × 5000 |
| Anti-hamster IgG HRP | Jackson ImmunoResearch, 127035160 | × 5000 |
| Western blot | ||
| Caspase-3 | Cell Signaling Technology, 9662 | × 1000 |
| Cleaved Caspase-3 (Asp175) | Cell Signaling Technology, 9661 | × 1000 |
| β-actin, AC-15 | Merck, A5441 | × 5000 |
| THBS-1 antibody, D7E5F | Cell Signaling Technology, 37879 | × 1000 |
| Anti-mouse IgG HRP | BioLegend, 405306 | × 5000 |
| Anti-rabbit IgG HRP | BioLegend, 406401 | × 5000 |