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. 2021 Jun 29;12:4028. doi: 10.1038/s41467-021-24282-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b UDM detergent molecules bound by the juxtamembrane helix. Simulated annealing 2F_o–F_c composite omit map contoured at 1.0σ. c A sulfate ion bound between JM and helix–turn–helix motif with F_o–F_c omit map contoured at 5.0σ. d Conservation of acidic residues in the AHB domain. e Sequence conservation of conserved glutamates in the AHB.