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. 2021 Jun 25;14:3881–3894. doi: 10.2147/OTT.S311038

Table 1.

The Primers for Quantitative Real-Time Polymerase Chain Reaction, with β-Actin and U6 Used as the Control

Primer Sets Name Reverse Transcriptase Primers (5ʹ to 3ʹ) RT-qPCR Primers (5ʹ to 3ʹ)
hsa-mir-100 GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACAAGT F: CAGTGCAGGGTCCGAGGTAT
R: CGCGAACCCGTAGATCCGAA
hsa-mir-99a GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACAAGA F: CAGTGCAGGGTCCGAGGTAT
R: CGTATAACCCGTAGATCCGAT
U6 GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATA F: AGAGAAGATTAGCATGGCCCCTG
R: ATCCAGTGCAGGGTCCGAGG
HS3ST F: GGCTGGATTGGTACAGGAGC
R: CCACGATCAGCTTGGTGTCT
β-actin F: CTCCATCCTGGCCTCGCTGT
R: GCTGTCACCTTCACCGTTCC