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. 2021 Jun 16;12:653587. doi: 10.3389/fmicb.2021.653587

TABLE 3.

EPS status of B. breve strains.

Strain Predicted EPS status EPS status by assay
UCC2003 Positive Positive
UCC2003_EPSneg Negative (mutant) Negative
JCM7017 Positive Positive
JCM7017_EPSneg Negative (mutant) Negative
JCM7019 Positive Positive
215W447A Positive Positive
017W439A Unclear Positive
082W48 Unclear Positive
689b Unclear Positive
NCTC 11815 Negative Negative
139W4 Positive Negative
NCFB 2258 Negative Negative
180W83 Unclear Negative
B12L Unclear Negative

Strains were predicted in silico to be EPS producers or non-producers based on the presence of pGTF and chain length regulator genes. EPS status in vitro was evaluated using EPS sedimentation assays.