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. 2021 Jun 16;12:650788. doi: 10.3389/fimmu.2021.650788

Figure 2.

Figure 2

IL-2 and MHC-II may regulate NKG2D expression on iDNT cells. (A) A representative experiment shows that a group of iDNT cells expressed NKG2D molecules. (B, C) Nonregulatory CD4+ T cells were cocultured with mature bone marrow dendritic cells (CD86-MHC-II+ DCs). IL-2 (50 ng/mL, 100 ng/mL or 200 ng/mL) was added to the mixed lymphocyte reaction. After successful induction, the percentage of NKG2D on iDNT cells was detected using flow cytometry. (B) Histograms are representative of NKG2D expression on iDNT cells. (C) The bar graphs are representative of three independent experiments. (D, E) Immature DCs (CD86+MHC-II-), mature DCs (CD86+MHC-II+) or mature DCs with anti-MHC-II antibody (20 μg/mL) were cocultured with nonregulatory CD4+ T cells. IL-2 (20 ng/mL) was added to the mixed lymphocyte reaction. After successful induction, the percentage of NKG2D on iDNT cells was analyzed using flow cytometry. (D) Histograms are representative of NKG2D expression on iDNT cells. (E) The bar graphs are representative of three independent experiments.