Figure 4.

NKG2D enhances iDNT cell-mediated inhibition of B cell differentiation into plasma cells in vitro. Freshly sorted TCRαβ⁺CD3⁺CD4^-CD8^-NKG2D⁺ or TCRαβ⁺CD3⁺CD4^-CD8^-NKG2D^- iDNT cells were incubated with naïve B cells at a gradient ratio of 0.4×10⁵, 0.2×10⁵ or 0.1×10⁵ iDNT cells to 4×10⁵ B cells in 96-well round-bottom plates in B cell medium for 2 days. LPS (10 μg/mL) was added to the medium, and we analyzed the activation, differentiation and apoptosis of B cells. (A) Representative flow cytometry profiles of B cell differentiation into plasma cells in vitro. (B) The bar graphs are representative of three independent B-cell differentiation experiments. (C) Histograms are representative of B cell apoptosis at different ratios. (D) The bar graphs are representative of three independent B-cell apoptosis experiments. (E) The bar graphs are representative of three independent B-cell activation experiments. Student’s t-test was used to compare two independent variables (ns, not significant, *p < 0.05, **p < 0.01, and ***p < 0.001).