TABLE 1.
Primers developed for the multilocus analysis
| Locus | Primer name | Primer sequence | Melting temp. | Product size (bp) |
|---|---|---|---|---|
| Β‐1‐tubulin | BTUB‐F | 5'‐GCCCCGACAACTTTGTCTTT‐3' | 55℃ | 636 |
| BTUB‐R | 5'‐TCTTGGCGTCGAACATTTGC‐3' | 56℃ | ||
| Copper‐exporting ATPase | ATP‐F | 5'‐CTTCCATCGCAATGCTGGTT‐3' | 55℃ | 551 |
| ATP‐R | 5'‐TGATGTCATCGCCTTCGAGT‐3' | 55℃ | ||
| Phosphate carrier protein | PHCP‐F | 5'‐CAGCAATCATGTCCGACAGA‐3' | 54℃ | 664 |
| PHCP‐R | 5'‐CGAACTTGGCCATGGTGTA‐3' | 54℃ | ||
| Topoisomerase‐1 | TOP1‐F | 5'‐CGCACTTCTCAAGGCTGGTAAT‐3' | 57℃ | 380 |
| TOP1‐R | 5'‐GGACGTCAAGCCGAATGTCA‐3' | 57℃ |