Figure 1.

SARS‐CoV‐2 ORF9b suppresses SeV‐ or poly (I:C)‐induced interferon β (IFN‐ β), IFN‐λ1, ISG56, and CLXL10 production. Plasmids of the pcDNA6B empty vector (500 ng) or SCV2‐ORF9b (500 ng) were transfected into HEK‐293T cells. Twenty‐four hours later, the cells were infected with SeV (A) or transfected with poly (I:C) (B) as indicated, and 9 and 12 h after stimulation, the expression of IFN‐β, IFN‐λ1, ISG56, and CLXL10 in these cells was determined by RT‐qPCR analysis. The results from one representative experiment are shown to represent three independent biological replicates. The error bars indicate the SD. EV, empty vector; RT‐qPCR, real‐time quantitative polymerase chain reaction; SARS‐CoV‐2, severe acute respiratory syndrome coronavirus 2; SCV2, SARS‐CoV‐2