Figure 6.

SARS‐CoV‐2 ORF9b suppresses the phosphorylation of TBK1 and IRF3. HEK‐293T cells were transfected with plasmids of (A) RIG‐IN (A), (B) MAVS, (C) TRIF, or (D) STING in the presence or absence of ORF9b for 24 h. The cells transfected with STING plasmid were further stimulated by the transfection of 2′3′‐cGAMP for 8 h. The transfected or stimulated cells were lysed and subjected to immunoblot analysis with the indicated antibodies. IRF3, IFN regulatory factor 3; SARS‐CoV‐2, severe acute respiratory syndrome coronavirus 2; TBK1, TANK‐binding kinase 1; TRIF, TIR‐domain‐containing adapter‐inducing interferon‐β