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Efficiencies of Atg7 knockdown by shRNAs. (A) Plasmids used for the transfection experiment. (B) Bright field (upper panels) and fluorescence (lower panels) images of NIH3T3 cells transfected with indicated shRNAs. The shCon indicates control shRNA. (C) Western blots showing expression levels of Atg7 in cells transfected with plasmids encoding indicated shRNA (1, shAtg7–1; 2, shAtg7–2; 3, shAtg7–3; 4, shAtg7–4).
