Fig. 2.

Inhibition of autophagy by Atg7 shRNA (A) Schematic illustration of the experimental procedure. Hep3B cells were transfected with plasmids expressing GFP-LC3 and shRNA and then subjected to starvation (B) Representative fluorescence images of Hep3B cells transfected with plasmids encoding indicated shRNA upon starvation. Cells were stained with DAPI to reveal nuclei (blue). Scale bar, 10 μm. (C) Western blots showing expression levels of LC3-I, LC3-II, and p62 in Hep3B cells depending on starvation (+ indicates starvation) and expression of shAtg7–2 (+ indicates shAtg7–2 expression).