Fig. 4.

Microscopic examination of liver tumors. (A) H&E and IHC image of paraffin-embedded sections from shCon and shAtg7–2 tumors. GFP was used as a reporter for shRNA expression. Scale bar, 200 µm. Inhibition of autophagy led to tumor suppression in the liver. (B) Representative images of Ki-67 staining in the shCon and shAtg7–2 tumors. Scale bar, 50 µm. (C) Quantification of nuclei positive for Ki-67 staining in tumors of each group. The values represent percentages of Ki-67-positivity among the total nuclei. The graphs represent the mean ± SEM. (**, p < 0.01).