miRNA-RT Poly(A) Tailing Reaction
| Reagent | 1X | Amount |
|---|---|---|
| Poly(A) Tailing Buffer | 5X | 2 μL |
| RNA | 1 μg | 7° - μL |
| RNase-free water | n/a | 7 – volume RNA |
| Poly A Polymerase | 1 μL | |
| Total | n/a | 10 μL |
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