PCR conditions (mRNA)
| PCR cycling conditions | |||
|---|---|---|---|
| Steps | Temperature | Time | Cycles |
| Initial Denaturation | 95°C | 03 min | 1 |
| Denaturation | 95°C | 05 sec | 40 cycles |
| Annealing | 58°C | 15 sec | |
| Extension | °C | 15 sec | |
| Final extension | 72°C | 5 min | 1 |
| Hold | 4°C | Forever | |
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