Figure 3.

Flow cytometric analysis of tdTomato-positive neural crest precursors in Tsc2^+/− reporter mice renal tumors

Representative flow cytometric data comparing single-cell events obtained between renal cortical tissue of Tsc2^+/+Mpz(Cre)TdT^fl/fl mice and Tsc2^+/−Mpz(Cre)TdT^fl/fl reporter mice at 4 months (A and B), 6 months (C and D), 12 months (E and F), and 16 months (G and H) are displayed, respectively. Single-cell suspensions of cranial neural crest cells (CNCCs) (I) and trunk neural crest cells (TNCCs) (J) excised from the 9.5dpc Tsc2^+/-Mpz(Cre)TdT^fl/fl mice embryos were used as positive controls to assess the emergence of tdTomato⁺ neural crest cells in tuberous sclerosis mice. Flow cytometric data shown is one representative experiment of doublet studies performed at different times using N = 2-3 mice/genotype/age group/experiment. Acquisition and analyses of flow cytometric data was performed on a Bio-Rad S3e cell sorter and analyzed using FlowJo 10.7.1/FCS Express 7 Plus software. All statistics were performed using a one-way ANOVA with post-hoc Dunnett test for multiple comparisons.