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. 2021 Jun 24;22(2):616. doi: 10.3892/ol.2021.12877

Figure 1.

Figure 1.

LGMN depletion attenuates the progression of thyroid carcinoma. (A) Reverse transcription-quantitative PCR analysis was conducted to determine the expression levels of LGMN in CAL-62 and SW579 cells transfected with sh-NC, sh-LGMN-1 or sh-LGMN-2. ***P<0.001. (B) Viability of CAL-62 or SW579 cells transfected with sh-NC, sh-LGMN-1 or sh-LGMN-2 was assessed by MTT assay. *P<0.05, **P<0.01. (C and D) Invasive ability of CAL-62 and SW579 cells transfected with sh-NC, sh-LGMN-1 or sh-LGMN-2 was evaluated by Transwell assay. ***P<0.001. (E and F) Tube length of HUVECs treated with conditioned medium from CAL-62 or SW579 cells transfected with sh-NC, sh-LGMN-1 or sh-LGMN-2. Magnification, ×200. ***P<0.001. Experiments were performed for three biological replicates. LGMN, pseudogene legumain; sh-NC, short hairpin RNA negative control.