Figure 3.

Subcellular localization of the translation initiation complex eIF4F

Proximity ligation assay was performed for eIF4E-eIF4G interaction followed by counterstaining the corresponding cellular organelles. For endoplasmic reticulum (ER) and Golgi staining, anti-Calnexin and anti-GalT antibodies were used, immunofluorescent probes (IF) with fluorofore Alexa488 were used for the visualization. For detecting mitochondria (Mito), a chemical probe Rhodamine 123 (Rh123) was used for the recognition of the mitochondria in the cells. Nuclear was counterstained with DAPI.