| Parameter name | Parameter value | Note |
|---|---|---|
| Objective | 20 X | Required for high throughput image acquisition |
| Objective | 63 X oil immersion | Required for high quality 3D quantification |
| Objective | 4 X | Required for the overview of the cell distribution and ROI selection. |
| Z-stack | 3 z-stacks with 1 μm for each focus plane | Required for high throughput image acquisition with 20 X objective |
| Z-stack | 20 z-stacks with 0.4 μm for each focus plane | Required for high quality 3D quantification with 63 X oil immersion objective |
| Filter | 405 nm/450nm | Required for DAPI nuclear staining visualization |
| Filter | 644 nm/670nm | Required for Cy5 far red PLA staining |
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