5 × protein sample buffer	Final concentration	Amount
2M Tris-HCL pH6.8	0.25 M	1.25 mL
SDS	10% (w/v)	1 g
2-mercaptoethanol	30% (v/v)	3 mL
Glycerol	50% (v/v)	5 mL
1% Bromophenol blue (BPB)	0.02% (w/v)	0.2 mL
Total		10 mL