Table 1. Digestion mix used in processing of L3-5 dorsal root ganglia (DRG) samples.
| Reagent (supplier, cat #) | Final
concentration |
|---|---|
| F12 (Gibco, 21765-029) | - |
| Dispase ll (Sigma Aldrich, 04942078001) | 3mg/ml |
| Collagenase type IA (Sigma Aldrich, C9891) | 12.5mg/ml |
| DNase l (Sigma Aldrich, 10104159001) | 10mg/ml |