Table 5. List of primers used to probe for genes associated with DNA damage, apoptosis and cellular stress.
| Gene | Function | Primer sequence |
|---|---|---|
| GAPDH forward | Housekeeping | GGCCTTCCGTGTTCCTAC |
| GAPDH Reverse | TGTCATCATACTTGGCAGGTT | |
| TRP53 forward | Cell cycle regulator, apoptosis inducible | GTCACAGCACATGACGGAGG |
| TRP53 reverse | TCTTCCAGATGCTCGGGATAC | |
| GADD45A forward | DNA damage/stress | CCGAAAGGATGGACACGGTG |
| GADD45A Reverse | TTATCGGGGTCTACGTTGAGC | |
| PUMA forward | Pro-apoptotic gene | GCGGCGGAGACAAGAAGA |
| PUMA reverse | AGTCCCATGAAGAGATTGTACATGAC | |
| FOS forward | Cell stress transcription factor | CGGGTTTCAACGCCGACTA |
| FOS reverse | TTGGCACTAGAGACGGACAGA | |
| SESN2 forward | Cell growth and survival regulator | TCCGAGTGCCATTCCGAGAT |
| SESN2 reverse | TCCGGGTGTAGACCCATCAC | |
| DRAM1 forward | DNA damage/autophagy | TCATCTCCTACGTGGTCGC |
| DRAM1 reverse | CTGCGCCAAGAAATGCAGAG | |
| MDM2 forward | P53 regulator | TGTCTGTGTCTACCGAGGGTG |
| MDM2 reverse | TCCAACGGACTTTAACAACTTCA | |
| PTEN forward | Cell growth regulator | TGGATTCGACTTAGACTTGACCT |
| PTEN reverse | GCGGTGTCATAATGTCTCTCAG | |
| F4/80 forward | Myeloid cells/macrophages | TGACTCACCTTGTGGTCCTAA |
| F4/80 reverse | CTTCCCAGAATCCAGTCTTTCC |