Table 2. Digestion mix used in processing of sciatic nerve and inguinal lymph node samples.
| Reagent (supplier, cat #) | Final
concentration |
|---|---|
| F12 (Gibco, 21765-029) | - |
| Collagenase type IA (Sigma Aldrich, C9891) | 6.25mg/ml |
| Pronase (Millipore, 53702) | 0.2% |
| Hyaluronidase (ABNOVA, P52330) | 0.4% |