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. 2021 Jun 11;6:68. Originally published 2021 Mar 29. [Version 2] doi: 10.12688/wellcomeopenres.16635.2

Table 2. Digestion mix used in processing of sciatic nerve and inguinal lymph node samples.

Reagent (supplier, cat #) Final
concentration
F12 (Gibco, 21765-029) -
Collagenase type IA (Sigma Aldrich, C9891) 6.25mg/ml
Pronase (Millipore, 53702) 0.2%
Hyaluronidase (ABNOVA, P52330) 0.4%