Table 4. Previously optimised ( Liang et al., 2020) antibody panel used for all flow cytometry experiments.
| Laser | Colour | Epitope | Cell Type | Final
Dilution |
Mono/
polyclonal |
Species | Cat # |
|---|---|---|---|---|---|---|---|
| UV | BUV395 | Ly6G | Neutrophils | 1:300 | Monoclonal | Rat anti-mouse | BD Bioscience,
#563978 |
| Violet | AmCyan | Live/
Dead |
- | 1:1000 | N/A | N/A | Invitrogen,
#L34959 |
| BV650 | Ly6C | Monocytes | 1:1500 | Monoclonal | Rat anti-mouse | BioLegend,
#128049 |
|
| Blue | FITC | CD45 | Leukocytes | 1:1200 | Monoclonal | Rat anti-mouse | BioLegend,
#103108 |
| Yellow | PE-Cy7 | CD11b | Myeloid lineage | 1:1200 | Monoclonal | Rat anti-mouse,
human |
BioLegend,
#101215 |
| PE | β -TCR | T cells (αβ
chain) |
1:300 | Monoclonal | Armenian hamster
anti-mouse |
BioLegend,
#109207 |
|
| Red | APC-Cy7 | MHCII | Activated
macrophages & dendritic cells |
1:1200 | Monoclonal | Rat anti-mouse | BioLegend,
#107628 |
| APC | δ-TCR | T cells (γδ chain) | 1:300 | Monoclonal | Armenian hamster
anti-mouse |
BioLegend,
#118116 |
|
| - | - | Fc block | CD16/32 | 1:20 | Monoclonal | Rat anti-mouse | BioLegend,
#101302 |